Parasexual Crosses by Hyphal Anastomosis and Protoplast Fusion in the Entomopathogen Verticillium lecanii
نویسندگان
چکیده
منابع مشابه
Production of extracellular enzymes in the entomopathogenic fungus Verticillium lecanii
This study investigates the mechanisms as well as strategies for purification and characterization of potential enzymes involved in pathogenesis of entomopathogenic fungi. The test strain of Verticillium lecanii that was screened, during the present investigation, proved to be an efficient producer of protein and polysaccharide degrading enzymes (amylase, protease, and lipase), hence indicating...
متن کاملGenetic Improvement of Fungal Pathogens
Potential Entomopathogenic Fungi a. Metarhizium spp. b. Beauveria spp. c. Verticillium lecanii d. Nomuraea rileyi e. Hirsutella spp. f. Paecilomyces spp. Genetic Improvement of EPF a) Chemical mutagenesis b) Parasexual cycle c) Protoplast fusion d) Direct genetic manipulation Mutagen In genetics, a mutagen is a physical or chemical agent that changes the genetic material, usually DNA, of an org...
متن کاملCharacterization of Spanish strains of Verticillium lecanii.
We have characterized biologically and physiologically eight Verticillium lecanii strains from several origins including insect pests. Of all the temperatures tested, 25 degrees C was the best for growth and at 40 degrees C none of the strains could grow. At 4 and 7 degrees C, growth was reduced in comparison to warmer temperatures. The strains had better development at pH close to 7 (F = 27.64...
متن کاملIntraspecific hybridisation of Trichoderma pseudokoningii by anastomosis and by protoplast fusion.
Double auxotrophic and morphological mutants of Trichoderma pseudokoningii Rifai were fused by anastomosis and by protoplast fusion. The recovery of recombinants from heterokaryons on different selective media and from heterokaryotic colonies indicated the occurrence of parasexual events. Prototrophic colonies growing on minimal medium produced binucleate spores, green in colour, revealing a no...
متن کاملEnhancement of Xylanase Production by Protoplast Fusion of Trichoderma spp.
Filamentous fungi Trichoderma spp. are efficient hyper-producers of industrially important enzymes. Xylanases are essential hemicellulases having vast ranges of applications in the various industrial sectors. In this study, the protoplast fusion of two T. virens and </span...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology
سال: 1987
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-133-12-3537